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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
29/09/2014 |
Actualizado : |
24/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
ISSN : |
0717-3458 |
DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
Idioma : |
Inglés |
Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
Thesagro : |
CITRUS; CYDIA. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02405naa a2200241 a 4500 001 1050705 005 2021-06-24 008 2012 bl uuuu u00u1 u #d 022 $a0717-3458 024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI 100 1 $aFEDERICI, M. 245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource] 260 $c2012 520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. 650 $aCITRUS 650 $aCYDIA 700 1 $aMARCONDES, J.A. 700 1 $aPICCHI, S.C. 700 1 $aSTUCHI, E.S. 700 1 $aFADEL, A.L. 700 1 $aLAIA, M.L. 700 1 $aLEMOS, M.V.F. 773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
20/03/2024 |
Actualizado : |
20/03/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
MACÊDO, I.L. DE; SOUSA, D.E.R. DE; SOUZA, A.D.R.; ARMIÉN, A.G.; MACHADO, M.; CASTRO, M.B. DE |
Afiliación : |
ISABEL LUANA DE MACÊDO, Veterinary Pathology and Forensic Laboratory, Veterinary Teaching Hospital, University of Brasilia, Distrito Federal, Brazil; Graduate Program in Animal Science, University of Brasilia, Distrito Federal, Brazil; DAVI EMANUEL RIBEIRO DE SOUSA, Veterinary Pathology and Forensic Laboratory, Veterinary Teaching Hospital, University of Brasilia, Distrito Federal, Brazil; Graduate Program in Animal Science, University of Brasilia, Distrito Federal, Brazil; ANTONIZETE DOS REIS SOUZA, Primatology Center, Fazenda Água Limpa, University of Brasilia, Distrito Federal, Brazil; ANÍBAL G. ARMIÉN, California Animal Health & Food Safety Laboratory System (CAHFS), School of Veterinary Medicine, University of California, Davis, USA; MIZAEL MACHADO DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MÁRCIO BOTELHO DE CASTRO, Veterinary Pathology and Forensic Laboratory, Veterinary Teaching Hospital, University of Brasilia, Distrito Federal, Brazil; Graduate Program in Animal Science, University of Brasilia, Distrito Federal, Brazil. |
Título : |
Fatal Africanized bee attack in captive non-human primates (Saimiri ustus and Sapajus libidinosus). |
Complemento del título : |
Short communication. |
Fecha de publicación : |
2024 |
Fuente / Imprenta : |
Toxicon. 2024, Volume 241, Article 107666. https://doi.org/10.1016/j.toxicon.2024.107666 |
ISSN : |
0041-0101 |
DOI : |
10.1016/j.toxicon.2024.107666 |
Idioma : |
Inglés |
Notas : |
Article history: Received 8 February 2024, Revised 26 February 2024, Accepted 27 February 2024, Available online 28 February 2024, Version of Record 8 March 2024. -- Correspondence: Castro, M.B.D.; Veterinary Pathology and Forensic Laboratory, Veterinary Teaching Hospital, University of Brasilia, Distrito Federal, Brazil; email:mbcastro@unb.br --Funding: This research was partly financed by the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES) - Finance Code 001 (ILM, DERS). We thank the National Council for Scientific and Technological Development ( CNPq ) for the Research Productivity grant (PQ), process n. 307909/2021-2 (MBC). |
Contenido : |
ABSTRACT.- Bee stings (BS) are a life-threatening issue and a growing concern for public health and animals in the Americas. We describe the clinical, pathological, and ultrastructural findings of a massive lethal bee attack in two non-human primates (NHPs). Both animals showed BS scattered throughout the skin, surrounded by a local reaction, diffuse pulmonary congestion, edema, hemorrhage, and remarkable degeneration and necrosis of renal epithelial cells from the proximal and distal tubules, characterizing a systemic bee envenomation reaction. © 2024 Elsevier Ltd |
Palabras claves : |
Bee venom; Envenomation; Kidney injury; Macaques; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Systemic reaction. |
Asunto categoría : |
L40 Estructura animal |
Marc : |
LEADER 02135naa a2200289 a 4500 001 1064505 005 2024-03-20 008 2024 bl uuuu u00u1 u #d 022 $a0041-0101 024 7 $a10.1016/j.toxicon.2024.107666$2DOI 100 1 $aMACÊDO, I.L. DE 245 $aFatal Africanized bee attack in captive non-human primates (Saimiri ustus and Sapajus libidinosus).$h[electronic resource] 260 $c2024 500 $aArticle history: Received 8 February 2024, Revised 26 February 2024, Accepted 27 February 2024, Available online 28 February 2024, Version of Record 8 March 2024. -- Correspondence: Castro, M.B.D.; Veterinary Pathology and Forensic Laboratory, Veterinary Teaching Hospital, University of Brasilia, Distrito Federal, Brazil; email:mbcastro@unb.br --Funding: This research was partly financed by the Coordination for the Improvement of Higher Education Personnel - Brazil (CAPES) - Finance Code 001 (ILM, DERS). We thank the National Council for Scientific and Technological Development ( CNPq ) for the Research Productivity grant (PQ), process n. 307909/2021-2 (MBC). 520 $aABSTRACT.- Bee stings (BS) are a life-threatening issue and a growing concern for public health and animals in the Americas. We describe the clinical, pathological, and ultrastructural findings of a massive lethal bee attack in two non-human primates (NHPs). Both animals showed BS scattered throughout the skin, surrounded by a local reaction, diffuse pulmonary congestion, edema, hemorrhage, and remarkable degeneration and necrosis of renal epithelial cells from the proximal and distal tubules, characterizing a systemic bee envenomation reaction. © 2024 Elsevier Ltd 653 $aBee venom 653 $aEnvenomation 653 $aKidney injury 653 $aMacaques 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aSystemic reaction 700 1 $aSOUSA, D.E.R. DE 700 1 $aSOUZA, A.D.R. 700 1 $aARMIÉN, A.G. 700 1 $aMACHADO, M. 700 1 $aCASTRO, M.B. DE 773 $tToxicon. 2024, Volume 241, Article 107666. https://doi.org/10.1016/j.toxicon.2024.107666
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